The Hutchison Lab Reverse Transcriptase Mutant Collection
Query the HIV-1 RT mutant database using Java Server Pages:
Residue Selection
Residue number *
Wild-type amino acid
Mutant amino acid
Amino Amino Change
All
A
C
D
E
F
G
H
I
K
L
M
N
P
Q
R
S
T
V
W
Y
All
A
C
D
E
F
G
H
I
K
L
M
N
P
Q
R
S
T
V
W
Y
All
Conservative
Non-conservative
display
yes
no
display
yes
no
display
yes
no
display
yes
no
* leave blank if you want to return all residues
Choose Mutant Phenotype Categories
RT activity
Total Western
Soluble Western
Western summary
Mutant Class
All
+++
++
+
-
All
wt
slightly reduced
US
UL
UL51
L
L51
I
neg
All
wt
slightly reduced
faint
very faint
no bands
n.d.
All
wt
non-wt
All
inactive
unstable1
unstable2
wt
display
yes
no
display
no
yes
display
no
yes
display
yes
no
display
yes
no
Choose Residue Classifications
Profiles3D environment
Genetically defined role
all
B1
B2
B3
P1
P2
E
all
CATALYTIC
FUNCTIONAL
functional
STABILITY-1
STABILITY-2
stability
EXTERNAL
display
no
yes
display
no
yes
Choose Amino Acid Properties
Class
Hydrophobic
Size
Polar
beta branch
wild-type amino acid
small
aliphatic
aromatic
basic
acid/polar
cysteine
yes
no
tiny
small
large
polar
non-polar
cysteine
beta-branch
non-beta-branch
mutant amino acid
small
aliphatic
aromatic
basic
acid/polar
cysteine
yes
no
tiny
small
large
polar
non-polar
cysteine
beta-branch
non-beta-branch
wild-type amino acid
display
no
yes
display
no
yes
display
no
yes
display
no
yes
display
no
yes
mutant amino acid
display
no
yes
display
no
yes
display
no
yes
display
no
yes
display
no
yes
Choose fields to sort by
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no sort
residue#
wt aa
mut aa
aa change
rt activity
total western
soluble western
western
mutant class
environment
role
no sort
residue#
wt aa
mut aa
aa change
rt activity
total western
soluble western
western
mutant class
environment
role
no sort
residue#
wt aa
mut aa
aa change
rt activity
total western
soluble western
western
mutant class
environment
role
no sort
residue#
wt aa
mut aa
aa change
rt activity
total western
soluble western
western
mutant class
environment
role